The uncoated liposomes displayed negative zeta potential. The biodistribution of various liposomes was performed after 24 h of intravenous administration in mice. Each group was consisted of 6 mice including 3 males and 3 females. The significant contribution of liposomes as drug delivery systems in. Cationic charge determines the distribution of liposomes. Read biodistribution characteristics of mannosylated, fucosylated, and galactosylated liposomes in mice, biochimica et biophysica acta bba general subjects on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. The livertumor uptake ratio of free drug, conventional ll, and sterically stabilized liposomes sll5000 and sll2000 was found to be 20, 7. Effect of liposome composition and other factors on the. Pegcoated phsensitive and pegfolatecoated phsensitive liposomes containing the gddtpabma complex were prepared and radiolabeled by neutron activation. The incorporation of doxorubicin in longcirculating sterically stabilized liposomes ssldxr alters the pharmacokinetics and biodistribution of doxorubicin and therefore has the potential to alter the pharmacologic properties of doxorubicin. Biodistribution of tat or qlpvm coupled to receptor. Biodistribution and pharmacokinetics of 188reliposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice chiache tsai1, chihhsien chang1, liangcheng chen1, yajen chang1, kengli lan2, yuhsien wu1, chinwei hsu1, ihsiang liu1, chungli ho1, wanchi lee1, hsiaochiang ni1, tsuijung chang1, gann ting3, tewei.
Biodistribution and pharmacokinetics of 111indtpalabelled. Lymphatic uptake from the sc injection site of small liposomes about 0. Tsai cc1, chang ch, chen lc, chang yj, lan kl, wu yh, hsu cw, liu ih, ho cl, lee wc, ni hc, chang tj, ting g, lee tw. View the article pdf and any associated supplements and figures for a period of 48 hours. The systemic pharmacokinetic studies were performed in rabbits. A strategy to prolong the blood circulation time of the liposomes includes the addition of a hydrophilic polymer polyethylene glycol peg moiety onto. Bresalier, and demetrios papahadjopoulos cancer research institute a. Characteristics and biodistribution of cationic liposomes. Trends and developments in liposome drug delivery systems. Pharmacokinetic, biodistribution and therapeutic efficacy of 5fluorouracilloaded phsensitive pegylated liposomal nanoparticles in hct116 tumor bearing mouse ofonime udofot1, kevin affram1, taylor smith1, bulumko tshabe1, sunil krishnan2,mandip sachdeva1, and edward agyare1. For biodistribution studies we used f9 teratocarcinoma tumours that strongly express edb fn in vivo, are highly vascularised and are not. Organ biodistribution of radiolabelled t cells following.
Effect of repetitiveadministration of doxorubicincontaining liposomes on plasma pharmacokinetics and drug biodistribution in a rat braintumor model robert d. Biodistribution and pharmacokinetics of 188reliposomes. We examined the biodistribution of indium 111labeled stealth liposomes in 17 patients with a variety of selected malignancies. For qualitative determination, mice were injected with lissamine rhodamine labeled liposomes and post 24 h of injection the whole mice body and various organs were imaged by an in vivo imaging system. Liposomal formulations have shown to accumulate in the target tissuewith enhanced biodistribution 28.
Biodistribution and pharmacokinetics of 111 indtpalabelled. Pdf preparation, pharmacokinetics and biodistribution of. Radiolabelling of antigen and liposomes for vaccine. Lymphatic uptake and biodistribution of liposomes after subcutaneous injection. For kinetic studies of dc614 liposomes, the injected dose was 2. To prepare chems liposomes egg pc, chems and mpegdspe 20. Each type of these agents small drugs, biologicals and ddss has advantages and challenges, some of which outlined in table 1. Tamra tmrpc liposomes and cy5dna within liver cryosections. M kamps and others published biodistribution and uptake of liposomes in vivo find, read and cite all the research you need on researchgate.
Biodistribution of tat or qlpvm coupled to receptor targeted. At 250 mg of iodine per kilogram, the rats had an average of 96 hu of hepatic and 321 hu of splenic enhancement. The biodistribution of the tagged liposomes confirms that increased radioactivity was seen in liver and spleen, with minimal involvement of the kidney. Biodistribution of liposomeencapsulated bacteriophages and their transcytosis during oral phage therapy jennifer otero, 1 alba garciarodriguez, 1 mary canosarabia, 2 daniel maspoch, 2, 3 ricard marcos, 1, 4 pilar cortes, 1, and montserrat llagostera 1. Eu compound has been prepared and incorporated into liposomes without alteration of its fluorescence signal. Nov 12, 2016 the method was developed to follow liposome biodistribution. Pdf preparation, characterization, and biodistribution.
Results characterization of lipid nanoparticles and evaluation of in vivo biodistribution we previously described a novel method of liposome formulation, the hydration of a freezedried matrix hfdm method. These applications are not considered in this chapter, nor are those that. Biodistribution and imaging studies1 alberto gabizon,2 david c. Accelerated blood clearance and altered biodistribution of. Preparation, biodistribution and neurotoxicity of liposomal.
Increased liver uptake of liposomes and improved targeting efficacy. The median halflife of 111inlabeled liposomes was 55 hours, with a pattern of biodistribution in the liver, spleen, and bone marrow consistent with uptake by. Cationic charge determines the distribution of liposomes between the vascular and extravascular compartments of tumors1 robert b. Liposomes, sphereshaped vesicles consisting of one or more phospholipid bilayers, were first described in the mid60s. Biodistribution of scfvliposomes in tumour bearing mice. Today, they are a very useful reproduction, reagent, and tool in various scientific disciplines, including mathematics and theoretical physics, biophysics, chemistry, colloid science, biochemistry, and biology.
Pegylated liposomes decorated with folic acid have been investigated for several anticancer agents not only to extend plasma halflife but also for tumor targeting via folic acid receptors which overexpressed on tumor cell surface. Jun 20, 2000 the biodistribution and pharmacokinetics of 111indtpalabelled pegylated liposomes in tumourbearing nude mice was studied to examine possible applications of pegylated liposometargeted anti. Biodistribution and pharmacokinetics of 188reliposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice chiache tsai1, chihhsien chang1, liangcheng chen1, yajen chang1, kengli lan2, yuhsien wu1, chinwei hsu1, ihsiang liu1, chungli ho1, wanchi lee1, hsiaochiang ni1, tsuijung chang1, gann ting3, tewei lee11institute of. If liposomes have favorable biodistribution, clearance, and imaging characteristics as. Preparation, pharmacokinetics and biodistribution of baicalinloaded. A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. The biodistribution and pharmacokinetics of stealth liposomes. Pdf preparation, characterization, and biodistribution study of. Previously, we showed that repetitive administration of ssldxr alters tumor vascular permeability.
Due to the complexity of in vivo environment, we studied the biodistribution of liposomes in mice and determined the ability of tfcpp liposomes in translocation across the bbb into the brain. Pharmacokinetics and biodistribution studies were carried out in rats after oral administration of balp and a carboxymethyl cellulose suspension containing ba bacmc as a control. Sterically stabilized liposomes are considered promising carriers of therapeutic agents because they can facilitate controlled release of the drugs, thereby reducing drugrelated toxicity andor targeted delivery of drugs. If liposomes have favorable biodistribution, clearance, and imaging characteristics as hepa tosplenic contrast agents. Cd inclusion complex ic was used to prepare ef24 liposomes. Effect of repetitive administration of doxorubicincontaining.
We compared linear dna with two types of lsnas that differ only by the affinity of the modified dna sequence for the liposome template. The nature and density of charge on the surface of the liposomes in. Pharmacodynamics and biodistribution of singledose liposomal. Biodistribution and pharmacokinetics of 188re liposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice chiache tsai1, chihhsien chang1, liangcheng chen1, yajen chang1, kengli lan2, yuhsien wu1, chinwei hsu1, ihsiang liu1, chungli ho1, wanchi lee1, hsiaochiang ni1, tsuijung chang1, gann ting3, tewei lee11institute of.
Biodistribution and pharmacokinetics of 188re liposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice. A strategy to prolong the blood circulation time of the liposomes includes the addition of a hydrophilic polymer polyethylene glycol peg moiety onto the surface of the vesicle. Pdf the purpose of this study was to prepare conventional and sterically stabilized liposomes containing leuprolide acetate in an attempt to. Modes of liposome action liposomes as drug delivery systems can offer several advantages over conventional. Cytotoxic targeting of f9 teratocarcinoma tumours with anti.
Slower drug release was observed in case of liposomes coated with acylated chitosans as compared to uncoated and native chitosan coated liposomes. Liposomal size was found to be the most important factor influencing lymphatic uptake and lymph node localization of sc administered liposomes. They are rapidly cleared from the circulation by the macrophages which are located mainly in the liver, spleen, and bone marrow. The biodistribution and pharmacokinetics of 111indtpalabelled pegylated liposomes in tumourbearing nude mice was studied to examine possible applications of. Biodistribution of liposomeencapsulated bacteriophages. Article metrics biodistribution and pharmacokinetics of. The radiolabeled liposomes presented significant in vitro cytotoxic activity against ehrlich.
Structuredependent biodistribution of liposomal spherical nucleic acids. After sc injection, the liposomes with a mean diameter of more than 100 nm were mostly accumulated at the injection site shown in figure 5a, following the recognition and metabolism by the local lymph nodes. It has been demonstrated that liposomes can increase drug solubility and stability as well as provide good drug loading for both hydrophilic and lipophilic drugs. Factors influencing ija formation in liposomes, namely icg. Jun 25, 2002 biodistribution of scfv liposomes in tumour bearing mice. Influence of peg coating on the biodistribution and tumor. For biodistribution studies we used f9 teratocarcinoma tumours that strongly express edb. Several nanomaterials, such as liposomes and iron oxide, gold, or silica nanoparticles, are already approved by the american food and drug administration for. Structuredependent biodistribution of liposomal spherical. Biodistribution of liposomes containing synthetic galactose. This work aims to quantitatively compare, and for the first time, the biodistribution profiles of human t cells in immunecompromised mice, implanted. Harrington from the cancer research uk targeted therapy laboratory, centre for cell and molecular biology, institute of cancer research, london, uk kn. Pharmacokinetic, biodistribution and therapeutic efficacy.
Interaction with and uptake of liposomes by liver and spleen macrophages can be substantially reduced, exploiting the properties of polyethylene glycol chains. Radiolabeling of leuprolide acetate and its liposomes was performed by direct labeling with reduced technetium99m. Pharmacokinetics, biodistribution, and pharmacodynamics of. Biodistribution and pharmacokinetics of 188reliposomes and. Biodistribution and pharmacokinetics of 111 indtpa. Today, they are a very useful reproduction, reagent, and tool in various. Scintigraphic visualization of intrathecal liposome. Influence of liposomal size, lipid composition and lipid dose. Tdb, has been extensively analysed in our lab 6,1518 and the results shown here were used to investigate the biodistribution of this liposome system as an adjuvant in vaccine studies. Add tags for accelerated blood clearance and altered biodistribution of repeated injections of sterically stabilized liposomes. Oct 26, 2011 biodistribution and pharmacokinetics of 188re liposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice chiache tsai1, chihhsien chang1, liangcheng chen1, yajen chang1, kengli lan2, yuhsien wu1, chinwei hsu1, ihsiang liu1, chungli ho1, wanchi lee1, hsiaochiang ni1, tsuijung chang1, gann ting3, tewei lee11institute of. Cytotoxic targeting of f9 teratocarcinoma tumours with. Lymphatic uptake and biodistribution of liposomes after. International journal of biological macromolecules.
The method was developed to follow liposome biodistribution. Herein, we studied the pharmacokinetics and biodistribution of repeated injections of radiolabeled polyethyleneglycol peg liposomes. Here, we investigate the biodistribution of liposomal spherical nucleic acid lsna conjugates, sna architectures formed from liposome templates and dna modified with hydrophobic end groups tails. In the present work, we report the development of a liposomal ija formulation, which was prepared in situ using lipid film hydration and extrusion. The dogs had 116 hu of hepatic and 65 hu of splenic enhancement. The way to proceed to use this method for liposomes or other vectors is detailed. Furthermore, the biodistribution and kinetics of nanomaterials and their modes of clearance are discussed, knowledge of the long. To determine the biodistribution of liposomes, mice were randomly divided into groups. Liposomes of quantum dots configured for passive and. The biodistribution and pharmacokinetics of stealth. Preparation, pharmacokinetics and biodistribution of baicalin. Applications of liposomes dr baumann cosmetics canada. Biodistribution and uptake of liposomes in vivo request pdf.
Pharmacokinetic, biodistribution and therapeutic efficacy of. Citations are the number of other articles citing this article, calculated by crossref and updated daily. Biodistribution of liposomes after extradural administration in rodents. Effect of liposome composition and other factors on the targeting of liposomes to experimental tumors.
Preparation, characterization, and biodistribution study. Effect of repetitiveadministration of doxorubicincontaining. Pharmacokinetic and biodistribution study in ehrlich ascites tumor bearing mice. Its biodistribution and imaging characteristics were studied in ehrlich ascites tumor eatbearing mice after labeling with technetium99m. Liposomes are lipid vesicles widely used as nanocarriers in targeted drug delivery systems for therapeutic andor diagnostic purposes. Balp exhibited a spherical shape by transmission electron microscopy observation. Read scintigraphic visualization of intrathecal liposome biodistribution, acta anaesthesiologica scandinavica on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The method has been validated by comparison with fluorophorelabeled liposomes. Liposometemplated indocyanine green j aggregates for. Pdf lymphatic uptake and biodistribution of liposomes. To this end, we have devised a dualradiolabelling method. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant and the antigen is favourable.
Biodistribution and pharmacokinetics of 111 indtpalabelled pegylated liposomes after intraperitoneal injection konstantinos n. Biodistribution of liposomes and dna liposome complexes male balbc mice weighing 2025 g from slc were administered the samples via tail vein. The median halflife of 111inlabeled liposomes was 55 hours, with a pattern of biodistribution in the liver, spleen, and bone marrow consistent with uptake by the reticuloendothelial system. The distribution of cy5labeled dna and tamralabeled liposomes 30 minutes post injection blue dapi nuclear stain, green phalloidin actin filament stain, red tmr tamrapc, magenta cy5dna. Efficient biodistribution and gene silencing in the lung. The biodistribution of liposomes in this model was observed using an in vivo imaging system, which showed that mgglgliposomes were present for significantly longer at the injection site compared to gglgliposomes. The incorporation of doxorubicin in longcirculating sterically stabilized liposomes. At 4h post injection the biodistribution data in kidney reveals approximately 12% of the injected dose was present for cyclosporine loaded liposomes, which elicits the possibility of reducing. Structuredependent biodistribution of liposomal spherical nucleic acids jennifer r. The biodistribution of aflabeled liposomes afl in mice and their incorporation into rat hepatocytes. Preparation, pharmacokinetics and biodistribution of baicalinloaded liposomes yumeng wei1, jianmin guo2, xiaoli zheng2, jun wu3 yang zhou1 yu yu 4 yun ye1 liangke zhang4 ling zhao1 1school of pharmacy, 2institute of basic medical sciences, luzhou medical college, luzhou city, sichuan.
If liposomes have favorable biodistribution, clearance, and imaging characteristics as hepatosplenic contrast agents. Preparation, pharmacokinetics and biodistribution of. Since then, liposomes have made their way to the market. Effect of repetitive administration of doxorubicin. M kamps and others published biodistribution and uptake of liposomes in vivo find, read and cite all the research. Biodistribution and pharmacokinetics of 188reliposomes and their comparative therapeutic efficacy with 5fluorouracil in c26 colonic peritoneal carcinomatosis mice. The aim of the present study was to develop a novel baloaded liposome balp to enhance oral bioavailability. The outstanding biological functions of the maleimidemodified liposomes as a novel drug delivery system make them ideally suited. These metrics are regularly updated to reflect usage leading up to the last few days. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals.
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